The Active Site of Bovine Plasminogen Activator
نویسندگان
چکیده
منابع مشابه
An active-site titrant for human tissue-type plasminogen activator.
The reaction of recombinant tissue-type plasminogen activator with the inverse substrate 4-amidino-2-nitrophenyl 4'-anisate results in the rapid release of the chromogen 4-amidino-2-nitrophenol and the accumulation of the relatively stable 4-anisoyl-enzyme. Spectrophotometric monitoring of the reaction enables the operational molarity of the enzyme to be determined.
متن کاملfunctionally active type 1 plasminogen activator inhibitor
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Induction of synthesis of the protease plasminogen activator (PA) by hormones, oncogenic viruses and tumor promoters occurs at the transcription level. A novel bioassay for PA messenger RNA was developed to study the regulation of PA synthesis and the genetic elements involved in it. Poly(A)containing RNA from HEp-3, a PA-rich tumor of human origin, was found to direct the synthesis of a new pr...
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The extracellular matrix (ECM) of cultured bovine aortic endothelial cells (BAEs) was analyzed by immunoblotting and reverse fibrin autography and shown to contain type 1 plasminogen activator inhibitor (PAI-1). Most PAI-1 in the ECM formed complexes with exogenously added tissue-type plasminogen activator (tPA), demonstrating that this PAI-1 was functionally active. The resulting tPA/PAI-1 com...
متن کاملMechanism of formation of bovine plasminogen activator from human plasmin.
It has been shown that the activation of bovine plasminogen by streptokinase plus a trace amount of human globulin involves a two-stage activation process (1, 2). Ablondi and Hagan (3) proposed that the proactivator of bovine plasminogen was identical with human plasminogen, which became activator by forming complexes with streptokinase. Kline and Fishman (4) using a lysine methyl ester assay d...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99335-x